If the two genes are unlinked, the probability of each genotypic combination is equal. To calculate the expected values, you can make a Punnett square. Step 1: Calculate the expected frequencies

This would suggest that the genes are linked.

Alternative hypothesis ( H a): The population of offspring do not have an equal probability of inheriting all possible genotypic combinations.

This would suggest that the genes are unlinked.

Null hypothesis ( H 0): The population of offspring have an equal probability of inheriting all possible genotypic combinations.

The hypotheses you’re testing with your experiment are: You perform a dihybrid cross between two heterozygous ( RY / ry) pea plants. Suppose that you want to know if the genes for pea texture (R = round, r = wrinkled) and color (Y = yellow, y = green) are linked. When genes are linked, the allele inherited for one gene affects the allele inherited for another gene.

One common application is to check if two genes are linked (i.e., if the assortment is independent). Chi-square goodness of fit tests are often used in genetics.

Graphpad prism 5 two way repeated anova